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KMID : 0379520010170010065
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한국독성학회지
2001 Volume.17 No. 1 p.65 ~ p.71
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Modification of Estrogenic Effect of Nonylphenol Combined with DEHP in Yeast-based Bioassay
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Park Mi-Sun
Jung Hai-Kwan
Park Hyun-Shin
Han Eui-Sik
Kim Jong-Won
Eom Mi-Ok
Jeong Sang-Hee
Oh Hye-Young
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Abstract
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The key targets of endocrine disruptors are nuclear hormone receptors, which bind to steroid hormones and regulate their gene transcription. A yeast-based steroid hormone receptor gene trascription assay was previously developed for the evaluation of chemicals with endocrine modulating activity. The yeast transformants used in this assay contain the human estrogen receptor along with the appropriate steroid response elements upstream of the beta-galactosidase reporter gene. We tried to evaluate several natural and synthetic steroids of their potential to interact directly with the steroid receptor. Some putative endocrine disruptors, including nonylphenol, are weakly estrogenic. But the combined treatment oj these chemicals with di-(2-ethylhexyl)phthalate (DEHP) significantly increased the beta-galactosidase activity in the yeast transformant. These results suggest that we also have to consider the synergistic effects of endocrine disruptors. In this study, we showed that yeast-based bioassay is a valuable tool for screening potential endocrine disruptors and quantitative determination of estrogenicity. And the possibility that the estrogen receptor binds multiple environmental chemicals adds another level of complexity to the interaction between the endocrine disruptors and the human hormone system.
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KEYWORD
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Human estrogen receptor, DEHR Nonylphenol, Synergistic effects, Yeast bioassay
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